What disinfectant to use against Senecavirus A? Does it vary based on surface type or temperature? These are the questions that a group of researchers from the University of Minnesota – Veterinary Diagnostic Laboratory are answering in the latest issue of the Journal of Swine Health and Production (JSHAP).

The project tested:
3 disinfectants: Household bleach, a phenolic disinfectant, and a quaternary ammoniun-aldehyde
5 surfaces: aluminium, stainless steel, rubber, cement, and plastic
2 temperatures: 25°C (77F) and 4°C (39F)

Summary

Objectives: To evaluate the virucidal efficacy of three commercial disinfectants against Senecavirus A (SVA) on five different surfaces at ~25°C and 4°C.

Materials and methods: Household bleach, a phenolic disinfectant, and a quaternary ammonium-aldehyde disinfectant were tested at manufacturer’s recommended concentrations against a contemporary strain of SVA on aluminum, stainless steel, rubber, cement, and plastic surfaces at ~25°C and 4°C. Virus propagation and titration were performed on swine testicular cells. Viral titers were calculated before and after exposure to the disinfectant being tested.

Results: At ~25°C, household bleach at 1:20 dilution inactivated ≥ 99.99% of the virus within 10 to 15 minutes on aluminum, rubber, and plastic. On stainless steel and cured cement, it inactivated 99.97% and 99.98% of the virus, respectively. At 4°C, bleach inactivated ≥ 99.99% of the virus within 5 to15 minutes on all surfaces except rubber; on rubber, inactivation was 99.91% after 15 minutes. The phenolic disinfectant at the manufacturer’s recommended concentration inactivated only ≤ 82.41% of the virus at either temperature and on any surface, even after a 60-minute contact time. Results for the quaternary ammonium disinfectant were intermediate: 78.12% to 99.81% of the virus was inactivated within 60 minutes at both temperatures and on all surfaces. To detect differences between disinfectants, paired Wilcoxon tests were performed. At 10- and 15-minute time points, efficacies of the three disinfectants differed significantly.

Implications: Significant variation exists in the antiviral efficacies of different disinfectants. Hence, they should be tested against various pathogens before use in the field.

Original article March 20, Pork Network

Efficacy of Three Disinfectants against Senecavirus A